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Transient Protein Expression Platforms
The evolution of transient protein expression with Expi platforms
At the research scale, transient protein expression platforms offer protein production labs many advantages over a stable system. However, this wasn’t always the case. Stable production used to be the only way to produce meaningful amounts of protein in a relatively short time period until in 2012 when Gibco launched Expi293 Expression System.
At the platform’s debut, the Expi293 transient expression platform offered up to 6x higher yield than previous transient systems and enabled protein harvest in as little as one week, which was weeks if not months faster than that achievable via stable development. Thermo Fisher Scientific has since continued to innovate upon the “Expi” platforms and launched ExpiCHO Expression System in 2015 and ExpiSf Expression System in 2018. For researchers and facilities needing to rapidly produce high amounts of protein, the Expi protein expression systems offer integrated solutions with yield, speed, and cost benefits.
Benefits of rapid, high-yield transient protein production
In addition to the obvious yield benefits offered by Expi platforms, labs can help minimize the burden on their staff by reducing hands-on-time needed for protein production, freeing up researchers to perform more experiments and analysis.
Speed
Stable cell line production can take almost a month; with Expi systems you can obtain sufficient yield to transiently express protein in as few as 5 days.
Yield
For researchers who need as much protein as possible, we've developed protocols that maximize yields without adding a significant time investment.
Cost-effectiveness
Since Expi systems offer high-yield and rapid expression, scientists can save on their cost/mg of protein (based on decreased plastics use, reduced staff time, and reduced DNA).
Transient protein expression systems by host type
Discover the benefits of our transient protein expression systems by host type.
*Note “Cost” above refers to a typical cost for a protein expression project in US dollars.
Transient protein expression systems by application
Not sure what system is right for you? Expand the links below to discover how transient protein expression is utilized in each application.
The Expi293 PRO Expression System enables a quick and efficient way to produce a high-yield of antigens for streamlining the development of human vaccines. The complete system enables gram-level protein yields in as little as 2–7 days, with optimized protocols allowing you to scale down to 96-well plates for automated and high-throughput platforms.
The ExpiSf Expression System accelerates vaccine development with the first chemically defined insect culture medium and integrated components designed to enable cost-effective and high-yield production of proteins and viral particles with consistent performance, run-after-run using a fast, streamlined, and highly-scalable workflow. With the simplicity, scalability, and cost-effectiveness of ExpiSf, you can get closer to your next vaccine breakthrough faster.
The Expi293 PRO Expression System enables high-yield expression of challenging and low-yield proteins, such as intracellular proteins and multiprotein complexes, using a transient HEK293 cell line. As an added benefit, the system’s fast kinetics allow for a faster, more streamlined workflow that gives you protein in 2–7 days.
The ExpiSf Expression System is a suitable and fast platform for high-titer production of these macromolecules leveraging an insect cell line.
The Expi293 Expression System has a suite of products specifically designed for glycan modulation, inducible expression, and methionine labeling, which help enable native folding and high protein yields required for structural biology applications. Whether your structural studies rely on X-ray crystallography or NMR, the Expi293 structural biology suite of products provide a complete, simple, and high performing system to help your work.
Learn more about the structural biology products
The ExpiSf Expression System can be an insect host, low-cost alternative platform for structural analysis where simple protein glycosylation is favorable.
The Expi293 PRO Expression System provides a platform for the fast and high-yield production of reagent proteins, such as enzymes, markers, receptors, and other functional proteins.
The ExpiSf Expression System offers an inexpensive path, through the use of an insect host cell line, for the transient production of reagent proteins.
The ExpiCHO Expression System comprises a family of integrated components that helps streamline biologics development by providing an end-to-end, integrated, and high-yield solution. Key quality risks are minimized during development by enabling the use of the same cell line from transient to stable production, while integrated reagents help streamline process development, reduce cost, and shorten timelines.
The Expi293 PRO Expression System is a fast and high-yield expression platform for the development of protein drug targets and antibodies. Because of its human glycan pattern, the Expi293 system is a superior choice for producing receptor proteins, kinases, GPCRs, and other target proteins of interest for potential drug therapies.
The Expi293 Met (–) Protein Labeling Kit includes Expi293 Met (–) Expression Medium and the ExpiFectamine 293 Met (–) Transfection Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labeling of methionine residues in proteins is desired. The kit components are specifically formulated without methionine, allowing users to add labeled methionine of their choice.
The Expi293 PRO Expression System is ideal for producing high-quality human-like proteins for the in vitro assessment of native protein interactions with therapeutic candidates. With its ability to deliver exceptional yields and express a wide variety of proteins, including challenging and low-expressing targets, you can generate the necessary reagents to investigate protein functions, interactions, and mechanisms. This system's efficiency and scalability ensure that sufficient quantities of active proteins are available for detailed and reliable functional assays, advancing our understanding of biological processes.
The ExpiCHO Expression System allows you to seamlessly transition from research to production scale by leveraging the same cell line as you progress from transient to stable production. When preparing for biologics development, it is important to start with a development platform that keeps your production plans in mind and enables you to save time and effort in scaling quickly and easily.
The Expi293 Expression System is another high-performing and flexible platform for transitioning from transient protein production to stable cell line development, whether you are looking to use stable cell lines to generate therapeutic proteins, leverage stable pools in early-stage drug discovery programs, or other applications.
Developing therapeutics against infectious diseases
Rapid protein production is essential for infectious disease research, where workflows must be optimized to generate high-quality proteins for structural research, serological testing, therapeutics discovery, and vaccine candidate screening.
In this white paper, learn how our optimized protein expression systems can help understand viral pathogenicity and combat rapidly evolving pathogens.
Sustainable solutions
We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
Protein Expression Publication Hub
The Protein Expression Publication Hub features a curated list of publications that offers an in-depth look into how scientists like you advance their research with Expi protein expression systems.
Our systems are optimized to harness the power of transient expression with the goal of delivering high-yield, consistent proteins for a diverse array of downstream applications. Search now
Resources
Application notes
Protocols
Scientific posters
Publications
Protein Expression Publication Hub
Access a curated list of publications that highlights the advantages of our Expi protein expression systems.
Related products
Protein Expression Learning Center
Access educational resources for better experiment planning and execution.
Support
Our Support Centers offer recommendations for your protein expression workflows, including tips for experimental setup and in-depth troubleshooting help.
For Research Use Only. Not for use in diagnostic procedures.