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Brightfield cell counting simplified

The Invitrogen Countess 3 Automated Cell Counter was developed using an advanced machine-learning image analysis algorithm to help deliver accurate cell counts and viability measurements in fewer than 30 seconds. The Countess 3 instrument has automated lighting, focus, capturing, counting, and saving; all you need to do is insert the slide containing a cell sample.

Features of the Countess 3 Automated Cell Counter

The Countess 3 Automated Cell Counter is accurate, precise, fast, simple, and automated. It offers viability measurement and single-cell data. It is compatible with reusable slides with optional software to support 21 CFR Part 11 compliance for electronic records.

Viability measurements via coulometric stains

Viability measurement using trypan blue

Murine PBMCs, often difficult to count due to their small size and low contrast, were counted with the Countess 3 Automated Cell Counter. In this brightfield count, Trypan Blue Solution 0.4% was used to distinguish viability, with live cells remaining translucent and dead cells staining dark. Red contours (circles) indicate cells counted as dead while green contours indicate cells counted as live. 

Viability measurement using SafeCount

Jurkat cells counted in brightfield mode on a Countess 3 Automated Cell Counter using SafeCount Cell Viability Stain. Red contours (circles) indicate cells counted as dead while green contours indicate cells counted as live.

Autofocus and autolighting on Countess 3

Autofocus can easily detect and focus on cells enabling accurate counts. Autolighting of both the brightfield image enables optimal images for detection of cells and clumps. Users can effortlessly adjust the focus and lighting for difficult samples and save the autofocus start point.

Countess 3 specifications

 

Technical specifications

Processing time: <30 seconds

Sample concentration range: 1 x 10⁴–1 x 10⁷ cells/mL

Particle diameter range: ~4–60 µm

Cell diameter range: ~4–60 µm

Required sample volume: 10 μL

USB drive: 32 GB (FAT32-, exFAT-, or NTSF-compatible)

Optics: 5 megapixel camera, 2.5X optical magnification

Physical characteristics

Instrument dimensions: 9" (W) x 5½" (D) x 9" (H); 228.6 mm (W) X 139.7 mm (D) x 228.6 mm (H)

Weight: 8 lbs; 3.6 kg

Counting area

Area: 2.26 mm x 1.69 mm (3.82 mm²)

Graphic image depicting the area of a typical standard hemocytometer and the area counted by the Countess 3 Cell counter which is significantly larger

Sold separately

Countess Reusable Slide

High-Power USB Wi-Fi Module (dongle)

Countess Cell Counting Chamber Slides

Trypan Blue Stain

Countess SAE Software Solution

Countess 3 Cell Counting Standard Slide

Countess 3 configuration options

 

Countess 3

Countess 3 Starter Package

Countess 3 Automated Cell Counter

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Power Cord with 4 adapters (for US/Canada/Taiwan/Japan, Europe, or UK)

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Countess Cell Counting Chamber Slides, 50 slides/box

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Countess Cell Counting Chamber Slides, 50 slides/box, 10 each

 

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Countess 3FL Disposable Slide Holder, 1 each

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Countess 3FL Reusable Slide Holder, 1 each

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Countess Reusable Slide

 

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Trypan Blue, 20 x 1 mL vials

 

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SafeCount, 4 x 1 mL vials

 

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Wi-Fi Dongle, 1 each

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Countess 3 USB Drive, 1 each

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Software updates

Ordering information

For Research Use Only. Not for use in diagnostic procedures.