Countess 3 FL Automated Cell Counter

Dual fluorescence and brightfield cell counting
 

The lnvitrogen Countess 3 FL Automated Cell Counter has fluorescence capabilities with two easily interchangeable fluorescent light cubes selected from 13 spectrum-spanning options. The Countess 3 FL Automated Cell Counter can count cells and measure viability in fluorescent mode as well as brightfield, while also reporting fluorescent protein expression, apoptosis, transfection efficiency, and cell viability using fluorescent reagents in as few as 30 seconds. Fluorescence cell counting also allows the use of fluorescence-based cellular assays that offer more specificity with complex samples by using fluorescent cellular stains or fluorescently tagged cells. With the utilization of artificial intelligence during development, our team has applied deep-learning neural network algorithms to generate highly accurate cell counts, even for the most challenging cell sample types.

The Countess 3 FL Automated Cell Counter offers integrated fluorescence capabilities, going beyond brightfield counting to support advanced assays and expanded applications—while enabling fast, accurate results through a simple, fully automated workflow. The system supports reliable viability measurements and detailed single-cell data across diverse cell lines, supports reusable slides, and offers optional software to help enable 21 CFR Part 11 compliance for electronic records.

Fluorescence counting applications

Go beyond cell counting with the integrated fluorescence capabilities of the Countess 3 FL Automated Cell Counter. Easily assess cell viability, apoptosis, transfection efficiency, and fluorescent protein expression—while having the flexibility to analyze virtually any cellular parameter that is autofluorescent or dye-labeled. Explore our Sample Data to see real-world examples, including cell culture quality control, CAR-T cell screening, CRISPR-Cas9 editing efficiency, and other powerful fluorescence-based applications.

Detect more with fluorescence-based counting

Increased sensitivity enables identification of lower-intensity signals, with flexible gating to refine results. The fluorescence-based counting mode expands flexibility by identifying and counting objects directly from fluorescent images, rather than relying on brightfield analysis. This approach can detect lower-intensity fluorescent objects, increasing sensitivity and enabling more comprehensive analysis—while still allowing users to gate out unwanted signals.

Because brightfield and fluorescence-based counts analyze samples differently, results such as object counts and size measurements may vary, giving users complementary insights depending on their experimental needs.