Serology Reagents

Specialty Reagents Optimized for Use with Our Serology Products

Our tissue typing family offers a host of HLA testing reagents optimized for use with our HLA typing trays. These products include unique isolation reagents, staining and fixing reagents, as well as a variety of control reagents optimized for use with our serological HLA typing trays as well as some of our antibody tests.


B27 Monoclonal Antibody

The B27 Monoclonal Antibody reagents react specifically to the Class I HLA and is to be used in immuno-staining of human lymphocytes in whole blood. The fluorescent intensity of lymphocytes can be analyzed on a flow cytometer after erythrocytes are lysed and removed.

  • Confirm the presence of HLA B27 as well as all eight sub-types associated with ankylosing spondylitis
  • Requires no additional testing reagents or analysis software

Bulk Monoclonal Antibodies

Unconjugated HLA Monoclonal Antibodies are available for a variety of research applications. These antibodies are supplied in lyophilized form (100 µl). Specificity and concentration (10X) are determined by the microcytotoxicity assay using standard NIH conditions. Biotinylated and FITC-conjugated HLA Monoclonal Antibodies are also available.

These monoclonal antibodies are available for a wide variety of applications including flow cytometry, animal cell typing, tissue staining, and chimerism studies.

Note: All FITC conjugated and biotinylated monoclonal Antibodies provide 25 tests per vial.

  • Wide selection of HLA specificities
  • Well-characterized
  • Superior technical support strategies

Cytotoxic Controls

Designed for the microcytotoxicity assay, One Lambda cytotoxic controls are used to test various conditions from the viability of a lymphocyte preparation to the reactivity of complement. One Lambda cytotoxic controls are provided in frozen form in 1 ml aliquots.

  • Pre-screened and ready to use
  • No preparation required

Dynabeads™

Gentle and tube-based magnetic separation with Dynabeads is the technology of choice when you want to isolate high yields of pure, viable and functional cells. The cells are not exposed to the stress of going through a dense column, and there's no risk of your results being affected by the isolation method.

Reduce variability and get truly reliable and reproducible lab results on which to build your studies, diagnostic assays and therapeutic protocols.

 

  • Isolates high yields of pure, viable and functional cells
  • Reduces variability and provides reproducible results
  • Results not affected by the isolation method

FluoroBeads™ Isolation Reagents

FluoroBeads T and FluoroBeads B Isolation Reagents are supraparamagnetic particles coupled to monoclonal antibodies specific to either T or B lymphocytes. The end result is a pure T or B lymphocyte population in less than 10 minutes.

FluoroBeads feature a particle size less than one micron in diameter, minimizing cell membrane damage, facilitating the use of automation, and increasing fluorescence intensities.

 

  • Short isolation time: less than 10 minutes
  • Isolation performed at room temperature
  • High yield with 98% purity
  • Superior stability
  • Small particle size: less than one micron in diameter; facilitates the use of automation; minimizes cell membrane damage and offers greater florescence

FluoroQuench™ Staining/Quenching Reagent  

Our FluoroQuench reagents consists of fluorescent dyes and quench that have been combined into a unique cocktail to stain lymphocytes, quench background fluorescence, and stop the reaction in one simple step. FluoroQuench AO/EB is the last reagent dispensed in the microcytotoxicity assay. It stains both live and dead cells, quenches background fluorescence, and preserves the cells. No additional dyes are required.

  • Premixed and ready to use
  • Stains, quenches, and stops the reaction in one simple step
  • Provides consistently accurate results
  • Enhances performance of automated systems
  • Eliminates pre-labeling with CFDA (FQ AO/EB only)
  • Reduces handling of potentially hazardous reagents

Goat IgG Anti-Human Kappa

Goat IgG Anti-Human Kappa augments the cytotoxic reactivity of HLA antibodies, cytotoxic negative absorption positive (CYNAP) reactive antibodies, by at least 4 to 8-fold, depending on the titer of HLA serum. Goat IgG Anti-Human Kappa is used to increase the sensitivity of HLA antibody detection in the crossmatch test.

  • Available in 1 ml and 50 ml volumes
  • Protein concentration and antibody protein (mg/ml) have been determined

Lympho-Kwik™

Lympho-Kwik Isolation Reagents provide a unique method for isolating specific lymphocyte populations. Unlike standard density gradients, the Lympho-Kwik test utilizes a combination of monoclonal antibodies, complement, and a stable density gradient to isolate the desired lymphocyte population. The procedure provides high purity and viability, saving valuable time in the process. Three versions of the Lympho-Kwik product are available:

  • Mononuclear Cells
  • T Lymphocytes
  • T and B Lymphocytes
  • Superior purity and viability 
  • Isolates specific lymphocyte populations
  • Available frozen
  • Great "clean-up" method
  • Easy procedure
  • Saves time

Pooled Human Serum

Designed for use as a cell culture protein supplement or as a negative control reagent.

PHS is derived from plasma collected from healthy AB blood group male donors at FDA-licensed facilities in the U.S. Individual plasma units are converted to serum and heat inactivated. The material is pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each unit is also tested and found negative for all required viral markers via FDA-approved methods.

  • Used as a cell culture protein supplement or a negative control reagent
  • Derived from plasma collected from healthy AB blood group male donors at FDA-licensed facilities in the U.S.
  • Viral markers tested: HBsAG, HIV-1, HIV-2, HCV, HIV-1Ag o r HIV-1 NAT, ALT, and Syphilis

Rabbit Complement

Rabbit serum is the most effective source of complement for HLA typing and antibody screening. Complement effectiveness is related to the titer, and to complement factors and heterophile antibodies. Optimized for use with our Terasaki HLA Tissue Typing Trays, our Class I and Class II rabbit complements are used to prepare cytotoxicity assays. Our rabbit complement is screened for activity and cytotoxicity using highly selected lymphocyte panels and characterized antisera to minimize lot-to-lot variation.

  • Long-lasting lots 
  • Available frozen and lyophilized 
  • Optimizes reactivity 
  • Active with monoclonal as well as alloantibodies 
  • No cytotoxic background 
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