Our tissue typing family offers a host of HLA testing reagents optimized for use with our HLA typing trays. These products include unique isolation reagents, staining and fixing reagents, as well as a variety of control reagents optimized for use with our serological HLA typing trays as well as some of our antibody tests.
The B27 Monoclonal Antibody reagents react specifically to the Class I HLA and is to be used in immuno-staining of human lymphocytes in whole blood. The fluorescent intensity of lymphocytes can be analyzed on a flow cytometer after erythrocytes are lysed and removed.
Unconjugated HLA Monoclonal Antibodies are available for a variety of research applications. These antibodies are supplied in lyophilized form (100 µl). Specificity and concentration (10X) are determined by the microcytotoxicity assay using standard NIH conditions. Biotinylated and FITC-conjugated HLA Monoclonal Antibodies are also available.
These monoclonal antibodies are available for a wide variety of applications including flow cytometry, animal cell typing, tissue staining, and chimerism studies.
Note: All FITC conjugated and biotinylated monoclonal Antibodies provide 25 tests per vial.
Designed for the microcytotoxicity assay, One Lambda cytotoxic controls are used to test various conditions from the viability of a lymphocyte preparation to the reactivity of complement. One Lambda cytotoxic controls are provided in frozen form in 1 ml aliquots.
Gentle and tube-based magnetic separation with Dynabeads is the technology of choice when you want to isolate high yields of pure, viable and functional cells. The cells are not exposed to the stress of going through a dense column, and there's no risk of your results being affected by the isolation method.
Reduce variability and get truly reliable and reproducible lab results on which to build your studies, diagnostic assays and therapeutic protocols.
FluoroBeads T and FluoroBeads B Isolation Reagents are supraparamagnetic particles coupled to monoclonal antibodies specific to either T or B lymphocytes. The end result is a pure T or B lymphocyte population in less than 10 minutes.
FluoroBeads feature a particle size less than one micron in diameter, minimizing cell membrane damage, facilitating the use of automation, and increasing fluorescence intensities.
Our FluoroQuench reagents consists of fluorescent dyes and quench that have been combined into a unique cocktail to stain lymphocytes, quench background fluorescence, and stop the reaction in one simple step. FluoroQuench AO/EB is the last reagent dispensed in the microcytotoxicity assay. It stains both live and dead cells, quenches background fluorescence, and preserves the cells. No additional dyes are required.
Goat IgG Anti-Human Kappa augments the cytotoxic reactivity of HLA antibodies, cytotoxic negative absorption positive (CYNAP) reactive antibodies, by at least 4 to 8-fold, depending on the titer of HLA serum. Goat IgG Anti-Human Kappa is used to increase the sensitivity of HLA antibody detection in the crossmatch test.
Lympho-Kwik Isolation Reagents provide a unique method for isolating specific lymphocyte populations. Unlike standard density gradients, the Lympho-Kwik test utilizes a combination of monoclonal antibodies, complement, and a stable density gradient to isolate the desired lymphocyte population. The procedure provides high purity and viability, saving valuable time in the process. Three versions of the Lympho-Kwik product are available:
PHS is derived from plasma collected from healthy AB blood group male donors at FDA-licensed facilities in the U.S. Individual plasma units are converted to serum and heat inactivated. The material is pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each unit is also tested and found negative for all required viral markers via FDA-approved methods.
Rabbit serum is the most effective source of complement for HLA typing and antibody screening. Complement effectiveness is related to the titer, and to complement factors and heterophile antibodies. Optimized for use with our Terasaki HLA Tissue Typing Trays, our Class I and Class II rabbit complements are used to prepare cytotoxicity assays. Our rabbit complement is screened for activity and cytotoxicity using highly selected lymphocyte panels and characterized antisera to minimize lot-to-lot variation.