pH Gradient Buffers

Thermo Scientific™  Related applications: Industrial Chromatography

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

The pH gradient platform saves time in method development and facilitates method transfer to QA/QC for a wide range of MAb charge variants through a generic LC-based approach to MAb characterization.

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Description

pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

  • Robust, reproducible gradients
  • Predict biomolecule pH
  • Simple method optimization
  • Easily automated
  • Applicable to the majority of MAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

Thermo Scientific pH buffer concentrates used in the pH gradient platform can be purchased individually or as a pair, in quantities of 125mL or 250mL. For added convenience, the 125mL buffers can also be bundled with columns in a number of specifically preconfigured kits.

  • The CX-1 pH gradient starter kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 10µm, 4 x 250mm column
  • The CX-1 pH gradient high throughput kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5µm, 4 x 50mm column
  • The CX-1 pH gradient high resolution kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5µm, 4 x 250mm column


Documents

Manuals & protocols