Invitrogen™

Dynabeads™ His-Tag Isolation and Pulldown

Catalog number: 10103D
Invitrogen™

Dynabeads™ His-Tag Isolation and Pulldown

Catalog number: 10103D
Catalog Number
10103D
Unit Size
2 mL
Price (USD)
262.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
1,096.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10103D2 mL
262.00
Your Price:
***
10104D10 mL
1,096.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
This product is designed for his-tagged protein isolation. High selectivity for poly-histidine tags results in extremely low background levels. The new bead surface chemistry is Cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes! The protocol includes instructions for making the required buffers as well as methods for optimizing buffer composition. Dynabeads® His-Tag Isolation & Pulldown replaces Dynabeads® TALON™. Dynabeads® His-Tag Isolation & Pulldown has a new optimized binding chemistry that offers significant advantages over Dynabeads® TALON™ which were coated in the TALON™ chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels. Dynabeads His-Tag Isolation & Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc. Note: This product contains Dynabeads and a protocol only, and does not include buffers. The technology used for the discontinued Dynabeads® TALON™ was licensed from Clontech. TALON™ is a registered trademark of Clontech Laboratories Inc., USA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
40 mg⁄ml
Quantity
2mL
Shelf Life
36 months from date of manufacture
Diameter (Metric) Bead
1 µm
Final Product Type
His-Tagged Protein
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Ligand
NTA
Sample Type
Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts
Shipping Condition
Room Temperature
Form
Magnetic beads
Research Category
For Research Use Only
Product Line
Dynabeads™

Contents & Storage

Dynabeads™ His-Tag Isolation & Pulldown beads are supplied as a suspension supplied in 20% ethanol. Store at: 2-8°C

Figures