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10503-027 - FAQs

View additional product information for 10503-027 - FAQs (10503-027)

2 product FAQs found

The DNAzol procedure says to dissolve the genomic DNA in 8 mM NaOH. Will the 8 mM NaOH damage my DNA?

No, the 8 mM NaOH will not affect the DNA integrity. In fact, DNA is most stable at slightly alkaline pH (>7). You will find that the isolated DNA does not resuspend well in water and has even worse solubility in Tris buffer. (Water often has a pH of lower than 7 due to dissolved CO2 from the air. This slightly acidic water will actually cause degradation of your DNA!) The pH of the 8 mM NaOH is ~9 and can be easily adjusted with TE or HEPES once the DNA is in solution. (Over time, the solution becomes neutral upon exposure to air from dissolved carbon dioxide.)

Do I need to wash my cell pellet before DNAzol reagent treatment?

No. Washing is not necessary.