Bac-to-Bac® HT Vector Kit

Catalog number: 10584027

Invitrogen™  Related applications: Protein Expression

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The Bac-to-Bac® HT Vector is designed for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:

• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification
For Research Use Only. Not for use in diagnostic procedures.


Cleavage: TEV Protease Recognition Site
Promoter: Polyhedrin
Vector Type: pFastBac
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x)

Contents & storage

The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector. Store the vectors at +4°C. All components are guaranteed stable for 6 months when properly stored.


Manuals & protocols

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