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The Bac-to-Bac™ HT Vector is designed for use as part of the Bac-to-Bac™ Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:
• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification
• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
Polyhedrin
Product Type
Vector Kit
Cleavage
TEV Protease Recognition Site
Protein Tag
His Tag (6x)
Cloning Method
Restriction Enzyme/MCS
Vector
pFastBac
Product Line
Bac-to-Bac™
Contents & Storage
The Bac-to-Bac™ HT Vector Kit includes 10 μg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored.
Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored.
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