The Bac-to-Bac® HT Vector is designed for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:
• Strong polyhedrin promoter for protein expression • Three reading frames for simplified cloning • N-terminal 6xHis tag for simple purification of recombinant fusion proteins • TEV protease cleavage site for removal of the histidine tag following protein purification
For Research Use Only. Not for use in diagnostic procedures.
TEV Protease Recognition Site
Restriction Enzyme ⁄ MCS
Protein Tag or Fusion:
His Tag (6x)
Contents & storage
The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
Store the vectors at 2–8°C. All components are guaranteed stable for 6 months when properly stored.