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Gibco™

Bac-to-Bac™ HT Vector Kit

Catalog number:  10584027

Related applications:

Protein Expression

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Description

The Bac-to-Bac® HT Vector is designed for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:

• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cleavage: TEV Protease Recognition Site
Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: Bac-to-Bac™
Product Size: 1 kit
Promoter: Polyhedrin
Protein Tag or Fusion: His Tag (6x)
Vector Type: pFastBac

Contents & storage

The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector.

Store the vectors at 2–8°C. All components are guaranteed stable for 6 months when properly stored.