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Invitrogen
CD122 Monoclonal Antibody (TU27), FITC, eBioscience™
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FIGURE: 1 / 2
CD122 Antibody (11-1228-41) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD122 FITC (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
11-1228-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TU27
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This TU27 monoclonal antibody reacts with human CD122, which is also known as interleukin-2 receptor beta (IL-2R beta). This receptor is expressed on NK cells, activated T cells, some B cells, monocytes, and T cell leukemias. CD122 can also be detected during early NK cell commitment. IL-2R beta associates with the IL-2R alpha (CD25) or IL-15R alpha and common gamma chain (CD132) to form the high affinity IL-2 and IL-15 receptors, respectively. By itself, this membrane receptor can bind IL-2, but with intermediate affinity. The binding of IL-2 results in internalization of the cytokine, tyrosine phosphorylation of IL-2Rbeta, and activation of signaling cascades such as the JAK/STAT pathway.
Applications Reported: This TU27 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TU27 antibody has been pre-titrated and tested by flow cytometric analyis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IL2RB; interleukin 15 receptor, beta; interleukin 2 receptor beta chain precursor peptide; interleukin 2 receptor, beta; interleukin-15 receptor subunit beta; p75; unnamed protein product
Gene Aliases: CD122; IL15RB; IMD63; P70-75
UniProt ID: (Human) P14784
Entrez Gene ID: (Human) 3560
signal transduction
JAK-STAT cascade
immunoglobulin mediated immune response
cytokine-mediated signaling pathway
interleukin-15-mediated signaling pathway
interleukin-2-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of phagocytosis
protein-containing complex assembly
positive regulation of leukocyte proliferation
positive regulation of leukocyte differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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