Opti-MEM™ I Reduced Serum Medium, no phenol red
Opti-MEM™ I Reduced Serum Medium, no phenol red
인용 및 참조 문헌 (2)
Gibco™

Opti-MEM™ I Reduced Serum Medium, no phenol red

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum자세히 알아보기
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카탈로그 번호수량
11058021500 mL
카탈로그 번호 11058021
제품 가격(KRW)
86,000
온라인 행사
Ends: 31-Dec-2025
90,000
할인액 4,000 (4%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
86,000
온라인 행사
Ends: 31-Dec-2025
90,000
할인액 4,000 (4%)
Each
카트에 추가하기

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I Reduced-Serum Medium modifications for a range of cell culture applications.


This Opti-MEM™ is modified as follows:
WithWithout
• L-glutamine• Phenol Red


The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ Reduced-Serum Medium
Opti-MEM™ I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양
세포주Sp2, AE-1, CHO, BHK-21, and HEK
세포 유형Primary Fibroblasts
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco, Opti-MEM
제품 유형Opti-MEM I
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Human Origin
형태Liquid
멸균Sterile-filtered
첨가제 포함Glutamine
첨가제 없음No Phenol Red
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

Do you offer an alternative for Opti-MEM I Reduced Serum Medium (Cat. No. 31985070)?

Opti-MEM I Reduced Serum Medium, no phenol red (Cat. No. 11058021) would be the closest alternative. Both offerings contain L-glutamine. For more a stabilized L-glutamine option, Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement (Cat. No. 51985034) is the recommended alternative. All Opti-MEM offerings have demonstrated performance for both reduced-serum cell culture and lipid-based transfection.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, no phenol red FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
L-phenylalanine and NPS R-467 synergistically potentiate the function of the extracellular calcium-sensing receptor through distinct sites.
Authors: Zhang Zaixiang; Jiang Yongfeng; Quinn Stephen J; Krapcho Karen; Nemeth Edward F; Bai Mei;
Journal:J Biol Chem
PubMed ID:12114500
The extracellular calcium (Ca(2+)(o))-sensing receptor (CaR) can be potentiated by allosteric activators including calcimimetics and l-amino acids. In this study, we found that many mutations had differential effects on the functional modulation of the CaR by these two allosteric activators, supporting the idea that these modulators act through distinct sites. ... More
Mutation of residues 423 (Met/Ile), 444 (Thr/Met), and 506 (Asn/Ser) confer cholesteryl esterase activity on rat lung carboxylesterase. Ser-506 is required for activation by cAMP-dependent protein kinase.
Authors: Wallace T J; Kodsi E M; Langston T B; Gergis M R; Grogan W M;
Journal:J Biol Chem
PubMed ID:11429416
Site-directed mutagenesis is used to identify amino acid residues that dictate reported differences in substrate specificity between rat hepatic neutral cytosolic cholesteryl ester hydrolase (hncCEH) and rat lung carboxylesterase (LCE), proteins differing by only 4 residues in their primary sequences. Beginning with LCE, the substitution Met(423) --> Ile(423) alone or ... More