Opti-MEM® I Reduced Serum Medium, no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 11058021

Gibco™  Related applications: Mammalian Cell Culture | Transfection

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Description

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I Reduced-Serum Medium modifications for a range of cell culture applications.

This Opti-MEM® is modified as follows:
WithWithout
• L-glutamine• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM® Reduced-Serum Medium
Opti-MEM® I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Opti-MEM® Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: No Phenol Red
Form: Liquid
Product Size: 500 mL
Volume (Metric): 500 ml
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

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Manuals & protocols

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