Dynabeads® M-280 Sheep Anti-Mouse IgG

Catalog number: 11202D

Novex™  Related applications: Antibody Purification | Chromatin Biology | Protein Assays and Analysis | Protein Purification & Isolation

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Description

Dynabeads® M-280 Sheep anti-Mouse IgG are 2.8 µm superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. These beads are designed to serve as a solid support for simple and efficient binding of mouse IgGs and their target proteins. The polyclonal sheep antibody binds both mouse IgG1, IgG2a, IgG2b, and with a lower reactivity towards mouse IgG3 and IgM. Human cross-reactivity is minimal.

• Fast and easy coupling and isolation procedure
• No columns or centrifugation steps required
• Binds most mouse IgG subclasses

Magnetic bead–based separation offers easy handling
The primary antibody that recognizes the target molecule may be added to the sample (indirect technique) or pre-coated onto the Dynabeads® M-280 Sheep anti-Mouse IgG (direct technique). Regardless of which of these techniques is used, when the Dynabeads® M-280 Sheep anti-Mouse IgG are mixed with the sample, the beads bind to the target. Placing the sample on a magnet separates the bead-bound target from the rest of the sample. The supernatant is then removed by aspiration. The target molecule can be eluted off the beads with conventional elution methods, or used in further downstream applications while still attached to the beads.

Adaptable for various applications
Dynabeads® M-280 Sheep anti-Mouse IgG are uniform and spherical, monodisperse, nonporous beads, making them an ideal choice for applications such as binding Ig, protein purification, sandwich immunoassays, IP, Co-IP, and isolation of cells and microorganisms. They are highly mobile in solution enabling the capture surface to freely and continuously interact with the entire sample volume.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Beads in Suspension
Cell Type: All cells from all species, except mouse
Reactivity: Mouse
Shelf Life: 36 months from date of manufacture
Ligand Type: Antibody
Host Species: Sheep
Label or Dye: Dynabeads® M-280
Product Size: 10 mL
Concentration: 10 mg⁄ml
Target Species: All species, except mouse
Antibody Fragment: Whole Antibody
High Throughput Compatibility: High Throughput-Compatible
Shipping Condition: Room Temperature

Contents & storage

Contains: 10 mL Dynabeads® M-280 Sheep anti-Mouse IgG (10 mg beads/mL)
Storage: 2°C to 8°C

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Manuals & protocols

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