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Ampicillin Sodium Salt (discontinued) -"DISCONTINUED" - FAQs

View additional product information for Ampicillin Sodium Salt (discontinued) -"DISCONTINUED" - FAQs (11593019)

2 product FAQs found

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

Can ampicillin be used for selection of eukaryotic cells if put under control of a eukaryotic/viral promoter?

No. B-lactamase is targeted to specific linkages in the bacterial cell wall. Since eukaryotic cells lack a cell wall, ampicillin has no effect upon eukaryotic cells.