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Gibco™
IMDM
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Description
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool. This IMDM is modified as follows:
| With | Without |
| • L-glutamine | • α-thioglycerol |
| • Phenol Red | • 2-mercaptoethanol |
The complete formulation is available.
Using IMDM
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
IMDM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical IMDM product made in our Scotland facility (21980-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Specifications
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| HEPES Buffer: | HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Glucose: | High Glucose |
| Product Line: | Gibco® |
| Product Size: | 6 x 1,000 mL |
| Volume (Metric): | 1000 ml |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
Contents & storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
