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Catalog number:  12440079

Related applications:

Mammalian Cell Culture

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Description

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This IMDM is modified as follows:
With Without
• L-glutamine • α-thioglycerol
• Phenol Red • 2-mercaptoethanol

The complete formulation is available.

Using IMDM
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
IMDM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical IMDM product made in our Scotland facility (21980-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Form: Liquid
Glucose: High Glucose
Glutamine: L-Glutamine
HEPES Buffer: HEPES
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: Sodium Pyruvate
Volume (Metric): 1000 ml
Quantity: 6 x 1000 mL

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations