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Invitrogen
CD135 (Flt3) Monoclonal Antibody (A2F10), Biotin, eBioscience™
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FIGURE: 1 / 7
CD135 (Flt3) Antibody (13-1351-82) in Flow
Stem cell research & therapy 2024 -
Fig. 4 NOD1 depletion corrects diabetes-induced hematopoietic imbalance. A Flow cytometry gating strategy for bone marrow single-cell suspensions. Viable/live singlets were gated on Lin - CD127 +- cells. Representative FACS plots of the analysis of HSPC subpopulations gated as LSK (Lin - CD127 - /Sca1 + c-Kit + ), LT-HSC (Lin - CD127 - /Sca1 + c-Kit + /CD34 - CD135 - ), ST-HSC (Lin - CD127 - /Sca1 + c-Kit +... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-1351-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
A2F10
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.
Applications Reported: The A2F10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: fetal liver kinase 2; FL cytokine receptor; flk-2; OTTHUMP00000214829; RP11-153M24.3; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; unnamed protein product
Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl
UniProt ID: (Mouse) Q00342
Entrez Gene ID: (Mouse) 14255
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
transmembrane signaling receptor activity
cytokine receptor activity
protein binding
ATP binding
growth factor binding
ubiquitin protein ligase binding
glucocorticoid receptor binding
phosphatidylinositol 3-kinase binding
macromolecular complex binding
leukocyte homeostasis
positive regulation of protein phosphorylation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
pro-B cell differentiation
pro-T cell differentiation
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
post-embryonic development
cell migration
cytokine-mediated signaling pathway
antigen processing and presentation
hemopoiesis
lymphocyte differentiation
B cell differentiation
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
common myeloid progenitor cell proliferation
dendritic cell homeostasis
positive regulation of multicellular organism growth
negative regulation of B cell differentiation
lymphocyte proliferation
protein autophosphorylation
lymph node development
spleen development
homeostasis of number of cells within a tissue
regulation of developmental process
cellular response to cytokine stimulus
dendritic cell differentiation
cellular response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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