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Invitrogen
Ly-49H Monoclonal Antibody (3D10), eBioscience™
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FIGURE: 1 / 4
Ly-49H Antibody (14-5886-37) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971) and 0.125 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714) (left) or 0.125 µg of Anti-Mouse Ly-49H Purified (right) followed by Anti-Mouse IgG Biotin and Streptavidin APC (Product # 17-4317).Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5886-37
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3D10
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
2 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D(Product # 12-5783-81) . Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.
Applications Reported:This 3D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3D10 antibody has been tested by flow cytometric analyis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytomegalovirus resistance 1; ly-49h; lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H; unnamed protein product
Gene Aliases: Cmv-1; Cmv1; Ly49h; Ly49u<129>
UniProt ID: (Mouse) Q60682
Entrez Gene ID: (Mouse) 16639
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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