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Invitrogen

IRF3 Monoclonal Antibody (SL-12 (SL12)), eBioscience™

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2 Published Figures
1 Reference
View all (75) IRF3 antibodies

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Product Details

14-9947-82

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
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Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SL-12 (SL12)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272269

Product Specific Information

Description: This SL-12 monoclonal antibody reacts with human Interferon Regulatory Factor (IRF)-3. IRF-3 is a 427 amino acid constitutively expressed member of the IRF transcription factor family that plays a critical role in the induction of type I interferons. Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Virus or dsRNA induces the C-terminal phosphorylation of IRF-3 by IKKε and TBK-1. This phosphorylation results in retention of IRF-3 in the nucleus and subsequent transcription of IFN-β, several interferon stimulated genes (ISGs), and other antiviral response genes.

Applications Reported: This SL-12 (SL12) antibody has been reported for use in immunoblotting (WB).

Applications Tested: This SL-12 (SL12) antibody has been tested by immunoblot of normal human peripheral blood cell lysates. This antibody can be used at less than or equal to 2 µg/mL in blocking buffer. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF-3a; IRF-3b; IRF-3c; IRF-3d; IRF-3e; IRF-3f; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3; unnamed protein product

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Gene Aliases: IIAE7; IRF3

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UniProt ID: (Human) Q14653

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Entrez Gene ID: (Human) 3661

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding identical protein binding protein homodimerization activity sequence-specific DNA binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production immune system process cytoplasmic pattern recognition receptor signaling pathway regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus cell surface receptor signaling pathway mRNA transcription response to bacterium regulation of gene expression programmed cell death signal transduction involved in regulation of gene expression lipopolysaccharide-mediated signaling pathway positive regulation of type I interferon production response to lipopolysaccharide positive regulation of interferon-alpha production positive regulation of interferon-beta production toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA innate immune response positive regulation of innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of inflammatory response defense response to virus type I interferon signaling pathway positive regulation of type I interferon-mediated signaling pathway cellular response to lipopolysaccharide cellular response to exogenous dsRNA macrophage apoptotic process programmed necrotic cell death cellular response to virus antiviral innate immune response cGAS/STING signaling pathway positive regulation of cytokine production involved in inflammatory response
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