Pierce™ Streptavidin Agarose Columns, 1 mL
Pierce™ Streptavidin Agarose Columns, 1 mL
Thermo Scientific™

Pierce™ Streptavidin Agarose Columns, 1 mL

Thermo Scientific Pierce Streptavidin Agarose Columns are 5-mL capacity columns containing 1 mL streptavidin agarose, for use in a varietyRead more
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Catalog number 20351
Price (USD)/ 5 columns
505.65
Online exclusive
528.00 
Save 22.35 (4%)
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Add to cart
Price (USD)/ 5 columns
505.65
Online exclusive
528.00 
Save 22.35 (4%)
Add to cart
Pierce™ Streptavidin Agarose Columns, 1 mL
Catalog number20351
Price (USD)/ 5 columns
505.65
Online exclusive
528.00 
Save 22.35 (4%)
-
Add to cart
Thermo Scientific Pierce Streptavidin Agarose Columns are 5-mL capacity columns containing 1 mL streptavidin agarose, for use in a variety of biotin-binding affinity purification methods

Features of Streptavidin Agarose:

Streptavidin—purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity—this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3 mg of biotinylated BSA per mL of resin

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar)

Applications:

• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
• Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
• Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing
• Streptavidin-coated plates used for direct solid-phase sequencing
In vitro mutagenesis with streptavidin-coated plates
• DNA fingerprinting with streptavidin-coated plates

Pierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

Related Products
Pierce™ Streptavidin Agarose
Pierce™ High Capacity Streptavidin Agarose
Pierce™ Streptavidin Plus UltraLink™ Resin
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity (Metric)5 mL
Particle Size45 to 165 μm
Product LinePierce™
Quantity5 Columns
Protein FormRecombinant
TargetBiotin-Tagged Molecule
TypeAgarose
Stationary PhaseStreptavidin
Sufficient ForBinding 15 to 28 μg Biotin Per Column (1 to 3 mg Biotinylated BSA Per Column)
FormatSpin Column
Column TypeAffinity, Agarose Resin
Unit Size5 columns
Contents & Storage
Contents: 5 × 1 mL prepacked columns with twist-off bottom tab and accessory pack containing five white tips.
Storage: Upon receipt store at 4°C. Do not freeze the resin.
Product is shipped at ambient temperature.