Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:
• Ready to use—carefully formulated and prefiltered 1X solutions • Three binding buffers—optimized for Protein A, Protein G or Protein A/G • Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral) • Versatile—elution buffers are appropriate for antibody-antigen affinity purification
Products available for each antibody purification method: • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide • IgG Elution Buffer: pH 2.8; amine-based • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)