Thermo Scientific EZ-Link Amine-PEG2-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods.
Features of EZ-Link Amine-PEG2-Biotin:
• Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules • Medium length—spacer arm (total length added to target) is 20.4 angstroms
Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.