Thermo Scientific™

EZ-Link™ Amine-PEG2-Biotin

Catalog number:  21346

 Related applications:

Protein Labeling & Crosslinking

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific EZ-Link Amine-PEG2-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods.

Features of EZ-Link Amine-PEG2-Biotin:

Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules
Medium length—spacer arm (total length added to target) is 20.4 angstroms

Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).

Related Products
EZ-Link™ Amine-PEG3-Biotin
For Research Use Only. Not for use in diagnostic procedures.


Cell Permeability: Cell-Impermeant
Spacer: Mid-Length, Pegylated
Label or Dye: Biotin
Chemical Reactivity: Aldehyde, Carboxylic Acid, Ketone
Product Line: EZ-Link™
Product Size: 50 mg
Solubility: Water

Contents & storage

Store at 4°C.


Manuals & protocols