Thermo Scientific™

EZ-Link™ Amine-PEG3-Biotin

Catalog number: 21347
Thermo Scientific™

EZ-Link™ Amine-PEG3-Biotin

Catalog number: 21347
Catalog Number
21347
Unit Size
50 mg
Price (USD)
Price: 371.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2134750 mg
Price: 371.00
Your Price:
-
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Thermo Scientific EZ-Link Amine-PEG3-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods.

Features of EZ-Link Amine-PEG3-Biotin:

Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules
Medium length—spacer arm (total length added to target) is 22.9 angstroms

Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).

Related Products
EZ-Link™ Amine-PEG2-Biotin
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Amine-PEG3-Biotin
Quantity
50 mg
Product Line
EZ-Link™
Cell Permeability
Cell-Impermeant
Chemical Reactivity
Carboxylic Acid, Ketone, Aldehyde
Reactive Moiety
Amine
Solubility
Water
Label or Dye
Biotin
Label Type
Biotin & Analogs
Spacer
Mid-Length, Pegylated

Contents & Storage

Store at 4°C.