DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Catalog number: 21578

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

DTSSP is 3,3'-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.

Features of DTSSP:

Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
• Cleavable crosslinker allows separation of crosslinked products
• Water-soluble; compare with DSP
• Membrane-impermeable, allowing for cell surface labeling

Applications:
• Chemical crosslinking of cell surface proteins prior to cell lysis and immunoprecipitation
• "Fix" protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Properties of DTSSP
• Alternative names: Sulfo-DSP
• Molecular formula: C14H14O14N2S4Na2
• Molecular weight: 608.51
• Spacer arm length: 12.0 Å (8 atoms)
• Reactive groups: Sulfo-NHS esters, react with primary amines at pH 7.0–9.0

DTSSP Specifications
We manufacture DTSSP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DTSSP is tested to meet the following minimum specifications:
Solubility: > 6 mg/mL in DI water
Identity: IR scan shows only peaks characteristic of the structure and functional groups of DTSSP
Purity: > 80% by quantitative NMR (the highest standard for crosslinker purity)

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))
DSP (dithiobis(succinimidyl propionate)), Lomant's Reagent
DSS (disuccinimidyl suberate)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Spacer: Long, Cleavable
Solubility: Water
Product Size: 50 mg
Labeling Method: Chemical Labeling
Crosslinker Type: Homobifunctional

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.

Documents

Manuals & protocols

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