DMEM, low glucose, GlutaMAX™ Supplement, pyruvate
Gibco™

DMEM, low glucose, GlutaMAX™ Supplement, pyruvate

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
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KatalognummerMenge
2188510810 x 500 ml
21885025
auch als 21885-025 bezeichnet
500 ml
Katalognummer 21885108
Preis (EUR)
201,65
Exklusiv online
232,00
Ersparnis 30,35 (13%)
Each
Menge:
10 x 500 ml
Customize this product
Preis (EUR)
201,65
Exklusiv online
232,00
Ersparnis 30,35 (13%)
Each
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

  • With: Low Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red
  • Without: HEPES

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. Gibco™ DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
ZelltypMammalian Cells
Konzentration1X
KulturmilieuCO2
Zur Verwendung mit (Anwendung)Mammalian Cell Culture
FormatBottles
Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard
ProduktlinieGibco, GlutaMAX
ProdukttypDMEM (Dulbecco's Modified Eagle Medium)
Menge10 x 500 ml
Haltbarkeit12 Monate
VersandbedingungAmbient
KlassifikationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormFlüssig
Serum LevelStandard Serum Supplementation
SterilitätSteril gefiltert
Sterilization MethodSterile-filtered
Mit AdditivenGlutaMAX, Geringer Glukosegehalt, Natriumpyruvat, Phenolrot
Ohne AdditiveKeine HEPES
Unit SizeEach
Inhalt und Lagerung
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Häufig gestellte Fragen (FAQ)

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.