Thermo Scientific™

LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))

Catalog number: 22362
Thermo Scientific™

LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))

Catalog number: 22362
Catalog Number
22362
Unit Size
50 mg
Price (USD)
Price: 208.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2236250 mg
Price: 208.00
Your price:
-
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Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

Features of LC-SMCC:

Reactive groups: NHS ester and maleimide
Reactive toward: amino and sulfhydryl groups
• Long-chain variety of SMCC
• High purity, crystalline reagent can be used to create high-purity maleimide-activated derivatives
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Cyclohexane bridge confers added stability to the maleimide group making LC-SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.

LC-SMCC is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of LC-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.

Applications:
• Ideal reagent for enzyme labeling of antibodies, both enzyme activity and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

LC-SMCC is often used for the preparation of antibody-enzyme and hapten-carrier conjugates. In this type of conjugation, the NHS ester is reacted first with the antibody, excess crosslinking reagent removed and then the sulfhydryl-containing enzyme molecule is added. This two-step reaction scheme results in formation of specific antibody-enzyme conjugates.

LC-SMCC is a reagent of choice for creating stable maleimide-activated carrier proteins that will spontaneously react with sulfhydryls. In this application, the NHS ester of LC-SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. These relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.

Specifications:
We manufacture LC-SMCC to the highest specifications to produce the most specific bioconjugates and maleimide-activated proteins, ensure the integrity of your data and to provide you with the highest degree of crosslinking consistency. Each lot of LC-SMCC is tested to meet the following minimum specifications.

• Identity: IR scan shows only peaks characteristic of the structure and functional groups of LC-SMCC crosslinker
• Purity: ≥ 90% by NMR

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Products
SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine-Sulfhydryl
Cleavable
No
Description
LC-SMCC
Molecular Weight (g/mol)
447.48
PEGylated
No
Spacer Arm Length
16.2 Å
Cell Permeability
Yes
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Maleimide, NHS Ester
Spacer
Medium (10 to 30 Å)
Form
Solid
Quantity
50 mg
Solubility
DMF, DMSO
Format
Standard
Water Soluble
No
Product Type
Crosslinker

Contents & Storage

Upon receipt store desiccated at 4°C.

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