Sulfo-SMCC (Sulfosuccinimidyll 4-(N-maleimidomethyl)cyclohexan-1-carboxylat), No-Weigh™ Format

Zitierungen und Referenzen (5)

Thermo Scientific™

Sulfo-SMCC (Sulfosuccinimidyll 4-(N-maleimidomethyl)cyclohexan-1-carboxylat), No-Weigh™ Format

Name: Sulfo-SMCC (Sulfosuccinimidyll 4-(N-maleimidomethyl)cyclohexan-1-carboxylat), No-Weigh™ Format
SKU: 22322
Price: 392.00 EUR

Ansicht ändern

Katalognummer	Menge
22322	50 mg
A39268	10 x 2 mg
22122	1 g

3 Optionen

Katalognummer 22322

Preis (EUR)

392,00

Each

Menge:

50 mg

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

392,00

Each

Thermo Scientific Pierce Sulfo-SMCC No-Weigh Format ist ein wasserlöslicher Amin-Sulfhydryl-Crosslinker, der NHS-Ester und maleimidreaktive Gruppen an gegenüberliegenden Enden eines mittellangen Cyclohexan-Spacerarms (8,3 Angström) enthält.

Merkmale von Sulfo-SMCC, No-Weigh Format:

• Reaktive Gruppen: Sulfo-NHS-Ester und Maleimid
• Reaktiv gegen: Amino- und Sulfhydrylgruppen
• Wasserlöslich (siehe SMCC), sodass die Vernetzung in physiologischen Lösungen erfolgen kann
• Hochreines, kristallines Reagenz kann verwendet werden, um hochreine maleimidaktivierte Derivate zu erzeugen
• Cyclohexan-Brücke verleiht der Maleimid-Gruppe zusätzliche Stabilität und macht Sulfo-SMCC zum idealen Verbindungsmittel für die Maleimid-Aktivierung von Proteinen. Maleimid-Gruppen sind in 0,1 M Natriumphosphatpuffer, pH 7 bei 4 °C 64 Stunden lang stabil.

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexan-1-carboxylat (Sulfo-SMCC) ist ein nicht spaltbarer und nicht membrangängiger Crosslinker. Er enthält ein aminreaktives N-Hydroxysuccinimid (NHS-Ester) und eine sulfhydrylreaktive Maleimidgruppe. NHS-Ester reagieren mit primären Aminen bei einem pH-Wert von 7 bis 9 und bilden stabile Amidbindungen. Maleimide reagieren mit Sulfhydrylgruppen bei einem pH-Wett von 6,5 bis 7,5 und bilden stabile Thioetherbindungen. Die Maleimid-Gruppen von Sulfo-SMCC und SMCC sind aufgrund der Cyclohexan-Brücke im Spacerarm ungewöhnlich stabil bis pH 7,5. Aufgrund seiner hydrophilen Sulfonylgruppe ist Sulfo-SMCC bis zu ∼10 mM in Wasser und vielen häufig verwendeten Puffern löslich, womit die Verwendung organischer Lösungsmittel, die eine Proteinstruktur stören können, vermieden wird.

Anwendungen:
• Enzymmarkierung von Antikörpern – sowohl Enzym- als auch Antikörperspezifität können erhalten werden
• Erstellen von spezifischen Biokonjugaten über ein- oder zweistufige Vernetzungsreaktionen
• Erstellen von sulfhydrylreaktiven, maleimidaktivierten Trägerproteinen für die Kopplung von Haptenen

Spezifikationen:
Sulfo-SMCC wird nach höchsten Anforderungen hergestellt, um die spezifischsten Biokonjugate und maleimidaktivierten Proteine zu produzieren, die Integrität Ihrer Daten zu gewährleisten und Ihnen ein Höchstmaß an Konsistenz bei der Vernetzung zu bieten. Jede Charge Sulfo-SMCC wird auf folgende Mindestanforderungen getestet.

• Identität: IR-Messung zeigt nur Peaks an, die für die Struktur und die funktionellen Gruppen der Sulfo-SMCC-Crosslinker charakteristisch sind
• Reinheit: ≥ 90% nach quantitativer NMR (der höchste Standard für Cross-Linker-Reinheit)
• Löslichkeit: ≥ 10 mg/ml in DI-Wasser

Produktreferenzen:
Crosslinker-Anwendungsleitfaden -- Suche nach aktuellen Literaturangaben für dieses Produkt

Ähnliche Produkte
Pierce™ Premium Grade Sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexan-1-carboxylat)
SM(PEG)4 (PEGylierter SMCC Crosslinker)

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZellpermeabilitätNein

BeschreibungSulfo-SMCC

FormPulver

MarkierungsmethodeChemische Markierung

Molekulargewicht436,37

PEGyliertNein

ProduktliniePierce

Menge50 mg

Reaktiver TeilMaleimid, Sulfo-NHS-Ester

VersandbedingungUmgebungstemperatur

LöslichkeitWasser

Länge des Spacer-Arms8,3 Å

WasserlöslichJa

Chemische ReaktivitätAmin-Sulfhydryl

CleavableNein

Crosslinker-TypHeterobifunktional

FormatStandard, Einmalgebrauch, Premiumqualität

ProdukttypCrosslinker

AbstandshalterKurz (<10 Å)

Unit SizeEach

Inhalt und Lagerung

Nach Erhalt getrocknet bei -20 °C lagern.

Häufig gestellte Fragen (FAQ)

How long is Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) functional group (maleimide) stable for after the amine targeted end is occupied?

The Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) maleimide group is stable for 64 hours in 0.1 M sodium buffer, pH 7 at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you provide the shelf-life for Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)?

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the shelf life of Sulfo-SMCC (Cat. No. 22322)?

Sulfo-SMCC is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between SMCC and Sulfo-SMCC?

SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). Sulfo-SMCC is a similar compound but it has a sulfonate group on the NHS ring which makes it water-soluble whereas SMCC is water-insoluble and needs to be dissolved first in an organic solvent like DMF or DMSO. Subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Sulfo-SMCC (Sulfosuccinimidyll 4-(N-maleimidomethyl)cyclohexan-1-carboxylat), No-Weigh™ Format FAQs

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E

Journal:Nucleic Acids Res

PubMed ID:11024189

Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More

Substrate-induced conformational change in bacterial complex I.

Authors:Mamedova AA, Holt PJ, Carroll J, Sazanov LA

Journal:J Biol Chem

PubMed ID:15037611

'The mechanism coupling electron transfer and proton pumping in respiratory complex I (NADH-ubiquinone oxidoreductase) has not been established, but it has been suggested that it involves conformational changes. Here, the influence of substrates on the conformation of purified complex I from Escherichia coli was studied by cross-linking and electron microscopy. ... More

Chondrex: new marker of joint disease.

Authors:Harvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C

Journal:Clin Chem

PubMed ID:9510855

Chondrex, a major secretory protein of human chondrocytes and synovial fibroblasts, is increased in serum of patients with joint and cartilage disease. We have developed a sandwich-type ELISA for quantifying chondrex in serum. The interassay CVs were 2.8-3.7% and the average within-run and total CVs were 3.6% and 5.4%, respectively. ... More

Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma.

Authors:Percy AJ, Trainor DA, Rittenhouse J, Phelps J, Koda JE

Journal:Clin Chem

PubMed ID:8605675

Amylin is a 37-amino-acid polypeptide synthesized in and secreted from pancreatic beta cells along with insulin. Its biological actions include the slowing and reduction of postmeal increases in plasma glucose concentrations. Studies of the basic amylin biology in humans have been hampered by the lack of a rapid, sensitive assay ... More

Sulfhydryl-based tumor antigen-carrier protein conjugates stimulate superior antitumor immunity against B cell lymphomas.

Authors:Betting DJ, Kafi K, Abdollahi-Fard A, Hurvitz SA, Timmerman JM

Journal:J Immunol

PubMed ID:18768870

Therapeutic vaccination of B cell lymphoma patients with tumor-specific Ig (idiotype, or Id) chemically coupled to the immunogenic foreign carrier protein keyhole limpet hemocyanin (KLH) using glutaraldehyde has shown promising results in early clinical trials, and phase III trials are underway. However, glutaraldehyde Id-KLH vaccines fail to elicit anti-Id immune ... More