Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Catalog number: PG82085

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Features of Sulfo-SMCC:

Sulfo-SMCC—a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
High quality—identity and purity confirmed by several tests, including quantitative NMR
Product integrity—enhanced level of testing and characterization compared to standard grade
Lot retention—ample supply of past lots retained to ensure future process testing
Change management—Change Control Notification (CCN) service
Consistent manufacture—batch-specific manufacturing documentation review

• Enzyme labelling of antibodies - both enzyme and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
For Research Use Only. Not for use in diagnostic procedures.


Spacer: Short
Solubility: Water
Product Size: 100 mg
Labeling Method: Chemical Labeling
Crosslinker Type: Heterobifunctional

Contents & storage

Upon receipt store desiccated at -20°C. Product is shipped at ambient temperature.


Manuals & protocols