Pierce™ Power Station - FAQs

View additional product information for Pierce™ Power Station - FAQs (22838)

6 product FAQs found

When I tried to stain my protein gel, it turned white? What happened?

Any staining procedure that uses over 50% alcohol will dehydrate a gel. Simply rehydrate the gel in some water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the Thermo Scientific Power Blot Cassette be used for staining and the Power Stain Cassette for blotting?

No. Each cassette can only be used for its intended purpose; Power Blot Cassette for blotting (fast protein transfer) and Power Stain Cassette for protein staining. We have optimized the surface area to yield the best results for each application. The smaller stain cassette works better for staining while the larger blot cassette works better for blotting.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the Thermo Scientific Power Station software, is it possible to insert the Power Blot Cassette and select the staining procedure?

No. It is not possible to insert the Power Blot Cassette and access the Power Staining protocol or vice versa.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will the Thermo Scientific Power Station automatically detect the Thermo Scientific Power Stain Cassette that is inserted?

Yes. The Thermo Scientific Power Station automatically recognizes the cassette that is inserted and initializes the correct software menu system.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the components of the Thermo Scientific Power System?

The Thermo Scientific Power System (Cat. No. 22830) consists of the Thermo Scientific Power Station (Cat. No. 22838) with activated Staining and Blotting Software, the Thermo Scientific Power Stain Cassette (Cat. No. 22836), and the Thermo Scientific Power Blot Cassette (Cat. No. 22835). It is a combination of the Power Stainer and the Power Blotter.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the components of the Thermo Scientific Power Stainer and how does it work?

The Thermo Scientific Power Stainer (Cat. No. 22833) consists of a Thermo Scientific Power Station (Cat. No. 22838) with activated Staining Software and a Thermo Scientific Power Stain Cassette (Cat. No. 22836). It is designed for rapid Coomassie staining of proteins in polyacrylamide gels and subsequent removal of stain from the gel background. Traditional Coomassie staining techniques require one hour to overnight of staining and destaining to achieve the desired results. When used in conjunction with Thermo Scientific Midi and Mini Gel Power Staining Kits, the Thermo Scientific Power Stainer is designed to provide staining efficiency in as few as 6 minutes that is equivalent to, or better than, traditional Coomassie staining techniques. This significant reduction in protein staining time is accomplished by fixing the protein to the gel and electrophoretically transporting the negatively charge Coomassie R250 dye rapidly through the gel matrix. The dye passes through the polyacrylamide and ionically binds to the protein, resulting in crisp blue bands with minimal background. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.