Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Catalog number: 23252

Thermo Scientific™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


This BCA Protein Assay Kit is the reducing agent-compatible version of our popular Thermo Scientific Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME), and comes with 20 96-well microplates.

Features of the Microplate BCA Protein Assay Kit—Reducing Agent Compatible:

Compatible—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
Small samples—requires only 25 µL (standard kit) or less than 10 µL (microplate kit) of sample
Colorimetric—measure with a standard spectrophotometer or plate reader (562nm)
Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
High linearity—linear working range for BSA equals 125 to 2000 µg/mL

The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. This reducing agent compatible (RAC) BCA Kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Related Products
Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible
96-Well Plates for Pierce™ BCA-RAC Assay
For Research Use Only. Not for use in diagnostic procedures.


Technique: Absorbance, Solution-based Detection
Assay Type: Protein Quantitation Assay
Product Size: 275 mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader, Spectrophotometer

Contents & storage

Sufficient For: 980 microplate assays with 9 µL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 48 x 10 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
• 96-Well Microplates, 20 plates

Store at room temperature


Manuals & protocols

Recommended products