Imperial™ Protein Stain

Catalog number: 24615

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

Thermo Scientific Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.

Features of Imperial Protein Stain:

Sensitive—detects less than 3ng protein per band with the enhanced protocol (3 hours)
Fast—ready-to-use reagent detects less than 6ng protein per band in just 20 minutes
Robust—highly consistent, reproducible protein staining technique
High contrast—intense purple bands are easier to photograph or scan than typical coomassie blue stains
Versatile—compatible with downstream mass spectrometry analysis and protein sequencing
Convenient—water washes only; no acid-fixative or methanol destaining solutions required
Stable—1-year room-temperature stability ensures consistent performance and saves refrigerator space
Flexible—adjust staining and washing times to meet time- and sensitivity-requirements

Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Imperial Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

Imperial Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements. The use of Imperial Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Imperial Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Molecule: Protein
Label or Dye: Coomassie
Detection Method: Colorimetric
Detection Location: In-Gel Detection, In-Blot Detection
Product Size: 1 L

Contents & storage

Upon receipt store product at room temperature.

Documents

Manuals & protocols