Thermo Scientific™

EZ-Link™ Alkoxyamine-PEG12-Biotin

Catalog number: 26139
Thermo Scientific™

EZ-Link™ Alkoxyamine-PEG12-Biotin

Catalog number: 26139
Catalog Number
26139
Unit Size
50 mg
Price (USD)
Price: 418.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2613950 mg
Price: 418.00
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Thermo Scientific EZ-Link Alkoxyamine-PEG12-Biotin is a biotinylation reagent containing a long, 12-unit polyethylene glycol (PEG) spacer for biotinylating macromolecules at carbohydrate groups that have been oxidized to form aldehydes.

Features of EZ-Link Alkoxyamine-PEG12-Biotin:

Glycoprotein labeling—biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
Cell surface labeling—biotinylate and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
Alkoxyamine-activated—aminooxy group forms more stable linkages than hydrazide reagents
Pegylated—spacer arm contains a hydrophilic, 12-unit, polyethylene glycol (PEG) group
Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
Irreversible—forms stable (permanent) oxime bonds; spacer arm cannot be cleaved
Solubility—usually dissolved in DMSO to make concentrated stock solution
Long reach—spacer arm (total length added to target) is 55.4 angstroms

Aminooxy-biotin reagents such as this one are useful for biotinylating glycoproteins and other molecules that have oxidizable polysaccharides groups. The alkoxyamine group (also called an aminooxy or aminoxy group) conjugates to aldehydes of oxidized sugars. EZ-Link Alkoxyamine-PEG-Biotin reagents contain a multi-functional extended spacer arm that is a flexible, non-immunogenic hydrophilic polyethylene glycol (PEG), which imparts water solubility to labeled molecules. Consequently, antibodies labeled with pegylated biotin reagents exhibit less aggregation when stored in solution compared to antibodies labeled with reagents having only hydrocarbon spacers.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Biotinylation reagents differ in reactivity, length, solubility, cell permeability and cleavability. Hydrazides and alkoxyamines are two types of carbonyl-reactive groups. Alkoxyamines (—O-NH2) react specifically with aldehyde groups in near-neutral conditions to form stable oxime linkages. The reaction is more efficient in the presence of aniline (Part No. 88944). Alternatively, alkoxyamines can be conjugated to carboxylic acids using EDC carbodiimide chemistry.

Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols using sodium periodiate (Part No. 20504). Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Alkoxyamine-PEG12-Biotin
Quantity
50 mg
Product Line
EZ-Link™
Cell Permeability
Cell-Impermeant
Chemical Reactivity
Ketone, Aldehyde
Reactive Moiety
Alkoxyamine
Solubility
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye
Biotin
Label Type
Biotin & Analogs
Spacer
Extra-Long, Pegylated

Contents & Storage

Store at -20°C. Shipped at ambient temperature.