Thermo Scientific™

Pierce™ 20X Modified Dulbecco's PBS Tween™ 20 Buffer

Catalog number:  28346

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Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is a space-saving stock solution used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays.

Features of 20X Modified Dulbecco's PBS Tween-20:

Dulbecco's PBS-T—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: Pierce™
Product Size: 500 mL
pH: 7.4 pH

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.


Manuals & protocols