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Opti-MEM™ I Reduced Serum Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  31985062

Related applications:

Cell Culture

| Cell Culture

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Description

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.

This Opti-MEM® I is modified as follows:
With
• L-glutamine
• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Scotland facility (31985-047). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Green Features: Sustainable packaging
Product Line: Gibco®, Opti-MEM®
Quantity: 100mL
Shipping Condition: Room Temperature
Volume (Metric): 100 ml
Form: Liquid
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture