Blocker™ Casein
Blocker™ Casein
Thermo Scientific™

Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.
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Catalog NumberQuantityBuffer
375321 LTBS
37582100 mLPBS
375281 LPBS
37583100 mLTBS
37528X33 x 1 LPBS
Catalog number 37532
Price (USD)
185.65
Online Exclusive
201.00
Save 15.35 (8%)
Each
Add to cart
Quantity:
1 L
Buffer:
TBS
Request bulk or custom format
Price (USD)
185.65
Online Exclusive
201.00
Save 15.35 (8%)
Each
Add to cart
Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.
Features of Blocker Casein include:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Blocking AgentSingle purified protein
BufferTBS
Concentration1X
For Use With (Application)Western Blot, ELISA
FormLiquid
Product LineBlocker™
Product TypeBlocker Casein
Quantity1 L
Unit SizeEach
Contents & Storage
Store at 4°C.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.