AmpFLSTR® NGM SElect™ PCR Amplification Kit

Catalog number: 4457890

Applied Biosystems™  Related applications: Forensics

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The NGM SElect™ Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect™ Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization. This enables the kit to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM SElect™ Kit:

• Exceptional data quality and discrimination capabilities support international data sharing
• Enables significantly enhanced performance on inhibited and degraded samples
• Enables greater sensitivity
• Maximizes concordance with previously typed samples
• Facilitates confident interpretation, promotes an optimized workflow and streamlines the implementation process

Data Quality Worth Sharing
The NGM SElect™ Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP), and includes the expanded European Standard Set of Loci (ESSL).

The NGM SElect™ kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel (Figure 1).

Configured for Success
Utilizing our expertise in 5-dye and mobility modifier technology, 17 loci are accommodated in a single amplification while maintaining optimum spacing (Figure 2). This permits unambiguous allele designation. All amplicons fall below 450 base pairs (bp) to maximize performance across a wide range of forensic samples. The majority of loci are concentrated below 200 bp, enabling recovery of a greater number of alleles from challenging degraded samples.

Powerful Discrimination
The combination of loci in the NGM SElect™ kit generates the highest power of discrimination of any AmpFLSTR® kit (Table 1). This helps minimize adventitious matches when comparing large data sets (e.g. for cross border data exchange). The NGM SElect™ kit also offers utility for other applications requiring higher levels of discrimination, including:
• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Permits Significantly Enhanced Performance on Inhibited and Degraded Samples
Leveraging the recent advances in PCR amplification technology pioneered with the NGM™ kit, the NGM SElect™ kit enables recovery of data from severely inhibited and degraded samples. For example, the NGM SElect™ kit is able to extract full profiles even from samples containing high concentrations of PCR inhibitors such as humic acid (Figure 3).

Performs with Greater Sensitivity
The NGM SElect™ Kit benefits from the same sensitivity enhancements as the NGM™ Kit, resulting in superior allele recovery at lower input levels (Figure 4). This can also enhance detection of the minor contributor in a mixed sample. The standard 29 cycle, 1 ng DNA input protocol enables maximum profile balance, and a validated 30 cycle protocol is also available. The higher cycle number leads to a corresponding increase in signal, increasing the possibility of allele detection from lower input DNA amounts. The use of 30 cycles also results in offscale data at an input of 1 ng (Figure 4); therefore, laboratories wishing to adopt this protocol should determine the optimum input DNA appropriate to their workflow through internal validation studies.

Maximizes Concordance
The NGM SElect™ Kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit as much as possible.

Facilitates Confident Data Interpretation
The NGM SElect™ kit benefits from improvements in primer synthesis techniques resulting in significantly cleaner baselines than previous AmpFLSTR® kits (Figure 5). The lack of dye related artifacts, even at 30 cycles, improves the ability of the analyst to interpret data, particularly for low level samples. Confident data interpretation is further enhanced by other improvements in profile quality, including:
• Enhanced sensitivity and robustness
• Maximized heterozygote and intracolor balance
• Optimum dye set utilization

These features also help facilitate streamlined processing of single source samples by expert system software such as GeneMapper® ID-X.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ kit has been designed with ease of implementation and use in mind. This is a single kit solution that delivers high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs.

The kit workflow is very similar to other AmpFLSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened by approximately one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, VIC®, FAM™, PET™, LIZ™
Product Size: 1,000 reactions
Amplicon Size: <450 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp™ 9700, 3500xL Genetic Analyzer, GeneAmp® 9600, 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3730 DNA Analyzer
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 LIZ® Size Standard
Shipping Condition: Dry Ice

Contents & storage

This kit contains AmpFLSTR® NGM SElect™ Primer Set, Master Mix, Allelic Ladder, and Control DNA 007. Store control DNA at 2 to 8°C. Other reagents should be stored at -15 to -25°C upon receipt, then at 2 to 8°C after first use. The kit contains enough reagents for 1000 reactions.


Manuals & protocols