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Granzyme A Monoclonal Antibody (GzA-3G8.5), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 2
Granzyme A Antibody (46-5831-82) in Flow
Balb/c splenocytes were cultured with Anti-Mouse CD3e and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82) for 3 days, then cultured with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) for an additional 5 hours. Cells were surface stained with Anti-Mouse CD8a FITC (Product # 11-0081-82) followed by intracellular staining with 0.06 µg of Mouse IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4732-82) (left) or 0.06 µg of Anti-Mouse Granzyme A PerCP-eFluor® 710 (right). Cells in th... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-5831-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
GzA-3G8.5
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This Gza-3G8.5 monoclonal antibody reacts with mouse Granzyme A. Granzyme A is the most abundantly expressed of the ten granzyme serine proteases that have been identified in mice. Granzymes are proteins released from the granules of NK cells and cytotoxic T lymphocytes that induce death in target cells by cleavage of intracellular substrates and play a critical role in immune defense against viruses, tumors, and intracellular bacteria. Granzyme A activates caspase-independent cell death pathways morphologically similar to apoptosis and characterized by mitochondrial and DNA damage. It may also play a role in inflammation, as the precursor form of IL-1 beta (pro-IL-1 beta) is among its target substrates. Granzyme A shares overlapping substrate specificity with the closely-related Granzyme K, which is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs.
Applications Reported: This GzA-3G8.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This GzA-3G8.5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: autocrine thymic lymphoma granzyme-like serine protease; BLT esterase; fragmentin-1; granzyme A precursor; H factor; Hanukah factor; preserine protease; serine esterase 1; t cell-specific serine protease 1; unnamed protein product
Gene Aliases: Ctla-3; Ctla3; Hf; Hf1; SE1; TSP-1; TSP1
UniProt ID: (Mouse) P11032
Entrez Gene ID: (Mouse) 14938
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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