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Invitrogen

CCL3 (MIP-1 alpha) Monoclonal Antibody (DNT3CC), PerCP-eFluor™ 710, eBioscience™

1 Published Figure
2 References
View all (50) CCL3 (MIP-1 alpha) antibodies

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Product Details

46-7532-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DNT3CC

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573842

Product Specific Information

Description: This DNT3CC monoclonal antibody reacts with mouse CCL3. CCL3, also known as MIP-1 alpha (Macrophage Inflammatory Protein 1 alpha), is a member of the CC- subfamily of chemokines and is most closely related to CCL4, or MIP-1 beta. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 will attract monocytes, macrophages, and dendritic cells, CCL3 preferentially attracts CD8+ T cells, while CD4+ T cells are more responsive to CCL4. In addition to its chemotactic functions, CCL3 induces inflammatory cytokine secretion, mast cell degranulation, and NK cell activation. It has also been reported to inhibit hematopoetic stem cell proliferation and may be responsible for the maintenance of these cells in a quiescent state. CCL3 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL4 and CCL5 (RANTES).

Applications Reported: This DNT3CC antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This DNT3CC antibody has been tested intracellular staining of stimulated mouse spleen cells followed by flow cytometric analysis using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: chemokine (C-C motif) ligand 3; H-MIP-1-alpha; heparin-binding chemotaxis protein; L2G25B; L2G25B protein precursor; LD78-alpha; macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1-alpha; MIP1 (a); mip1 alpha; Mip1a; RP23-320E6.7; SIS-alpha; small-inducible cytokine A3; TY-5; unnamed protein product

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Gene Aliases: G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3

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UniProt ID: (Mouse) P10855

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Entrez Gene ID: (Mouse) 20302

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Function(s)
protein kinase activity protein binding chemokine activity phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding CCR chemokine receptor binding
Process(es)
osteoblast differentiation cell activation monocyte chemotaxis calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response immune response cytoskeleton organization positive regulation of cytosolic calcium ion concentration cell-cell signaling regulation of cell shape response to xenobiotic stimulus response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration neutrophil chemotaxis leukocyte chemotaxis positive regulation of interleukin-1 beta production positive regulation of tumor necrosis factor production regulation of programmed cell death eosinophil degranulation positive regulation of neuron apoptotic process astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of calcium ion transport regulation of sensory perception of pain cell chemotaxis antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade response to cholesterol granulocyte chemotaxis positive regulation of natural killer cell chemotaxis
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