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Invitrogen

CXCL10 (IP-10) Monoclonal Antibody (4NY8UN), PerCP-eFluor™ 710, eBioscience™

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View all (36) CXCL10 (IP-10) antibodies

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Product Details

46-9744-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4NY8UN

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573897

Product Specific Information

Description: This 4NY8UN monoclonal antibody reacts with human CXCL10. CXCL10, also known as Interferon Inducible Protein-10 (IP-10), is a member of the CXC family of chemokines. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. CXCL10 signaling is mediated by the G protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. In addition to its chemotactic functions, CXCL10 is a potent inhibitor of angiogenesis and tumor growth and modulates expression of adhesion molecules. Elevated levels of CXCL10 are observed in response to allergic inflammation, various infections, and Alzheimer's disease.

Applications Reported: This 4NY8UN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4NY8UN antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; gamma IP10; Gamma-IP10; H-IP-10; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10

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Gene Aliases: C7; crg-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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UniProt ID: (Human) P02778

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Entrez Gene ID: (Human) 3627

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Function(s)
receptor binding cytokine activity protein binding chemokine activity heparin binding cAMP-dependent protein kinase regulator activity chemoattractant activity CXCR chemokine receptor binding CXCR3 chemokine receptor binding
Process(es)
positive regulation of leukocyte chemotaxis chemotaxis defense response inflammatory response immune response signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway cell-cell signaling muscle organ development blood circulation positive regulation of cell proliferation response to bacterium response to gamma radiation T cell chemotaxis regulation of T cell chemotaxis response to auditory stimulus negative regulation of angiogenesis positive regulation of cell migration neutrophil chemotaxis killing of cells of other organism response to lipopolysaccharide response to vitamin D cellular response to heat endothelial cell activation regulation of cell proliferation regulation of apoptotic process negative regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive chemotaxis positive regulation of release of sequestered calcium ion into cytosol defense response to virus antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway cellular response to lipopolysaccharide positive regulation of monocyte chemotaxis cellular response to interleukin-17 cellular response to virus antiviral innate immune response regulation of endothelial tube morphogenesis negative regulation of myoblast fusion positive regulation of T cell migration
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