DyLight™ 350 NHS Ester
DyLight™ 350 NHS Ester
Thermo Scientific™

DyLight™ 350 NHS Ester

Thermo Scientific DyLight 350 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 350 used to fluorescently label antibodiesRead more
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Catalog number 46426
Price (USD)/ 1 mg
371.00
-
Add to cart
Description:
DyLight 350 NHS Ester
Quantity:
1 mg
Price (USD)/ 1 mg
371.00
Add to cart
DyLight™ 350 NHS Ester
Catalog number46426
Price (USD)/ 1 mg
371.00
-
Add to cart
Thermo Scientific DyLight 350 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 350 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods.

DyLight 350 is a derivative of aminomethylcoumarine acetate (AMCA) and has a higher fluorescence intensity than Alexa Fluor™ 350 and AMCA in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 350 Amine-Reactive Dye is also available as part of two antibody labeling kit sizes.

Features of DyLight 350 NHS Ester:

High performance—DyLight 350 shows brighter fluorescence than Alexa Fluor 350 and AMCA
Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Applications
• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies

DyLight 350 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require DyLight 350 Amine-Reactive Dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 350 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 350 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods.The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 μg of IgG. Both kit sizes include the Amine-Reactive DyLight 350 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Related Products
DyLight™ 350 Antibody Labeling Kit
DyLight™ 350 Microscale Antibody Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDyLight 350 NHS Ester
Quantity1 mg
Product TypeDye
SolubilityDMF (Dimethylformamide)
Chemical ReactivityAmine
Reactive MoietyActive Ester, Succinimidyl Ester, NHS Ester
Label or DyeDyLight™ 350
Label TypeDyLight Fluors
Product LineDyLight™
Emission432
Excitation353
Labeling TargetProteins
Unit Size1 mg
Contents & Storage
Store desiccated at -20°C, protected from light.