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IL-2 Antibody (48-7029-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) and then intracellularly stained with Anti-Human CD4 APC (Product # 17-0047-42) and Anti-Human IL-2 eFluor® 450, using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

48-7029-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_2574101

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: This MQ1-17H12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ1-17H12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aldesleukin; cytokine; H-IL-2; il 2; ILN; Interleukin; interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; T cell growth factor; T-cell growth factor (TCGF)

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Gene Aliases: IL-2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

cytokine activity interleukin-2 receptor binding protein binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (MQ1-17H12), eFluor™ 450, eBioscience™

IL-2 Antibody (48-7029-42) in Flow

Product Details

48-7029-42

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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