Thermo Scientific™

CaptureSelect™ FcXP Pre-packed Column

Catalog number: 494371205
Thermo Scientific™

CaptureSelect™ FcXP Pre-packed Column

Catalog number: 494371205
Catalog Number
494371205
Unit Size
1 x 5 mL column
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
5 x 1 mL columns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4943712051 x 5 mL column
-
4943712015 x 1 mL columns
-
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CaptureSelect FcXP Affinity Matrix is offered here in a pre-packed format with connectors that are compatible with standard chromatography equipment.

CaptureSelect FcXP Affinity Matrix is the successor to CaptureSelect FcXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4). But the matrix features an increased binding capacity (>40 g IgG/L), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix.

Features of this affinity matrix include:

  • Mild elution, making it suitable for Fc fusion proteins
  • Human specific, no binding to bovine antibodies
  • Excellent scalability
  • Non-animal derived

CaptureSelect FcXP pre-packed columns can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enable a robust and efficient purification process with excellent purity in one step.

Characteristics:

  • Matrix: agarose-based, epoxide activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect FcXP affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl2, 40% (v/v) propylene glycol, pH 7
  • Flow characteristics: 150–300 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Binding Capacity
40 to 50 g IgG/L resin
Flow Rate
150 to 300 cm/hr. (Up to 2 bar)
Matrix
Agarose-based, epoxide-activated
Shipping Condition
Wet Ice
Particle Size
65 ± 10 μm
Product Line
CaptureSelect™
Quantity
1 x 5 mL Column
Type
Pre-packed Column
Ligand Type
FcXP affinity ligand

Contents & Storage

Store at 2°C to 8°C.

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