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CD115 (c-fms) Monoclonal Antibody (AFS98), PE-eFluor™ 610, eBioscience™
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FIGURE: 1 / 28
CD115 (c-fms) Antibody (61-1152-82) in Flow
C57Bl/6 bone marrow cells were cultured for 3 days with Mouse M-CSF Recombinant Protein (Product # 14-8983-80). Cells were stained with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.03 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.03 µg of Anti-Mouse CD115 (c-fms) PE-eFluor® 610 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
61-1152-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Fish,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AFS98
Conjugate
PE-eFluor™ 610
PE-eFluor™ 610
PE-eFluor™ 610
Excitation/Emission Max
565/606 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.
Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived macrophages. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-fms; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; OTTHUMP00000224093; proto-oncogene c-Fms; proto-oncogene fms; unnamed protein product
Gene Aliases: CD115; CSF-1R; Csfmr; Fim-2; Fim2; Fms; M-CSF-R; M-CSFR
UniProt ID: (Mouse) P09581
Entrez Gene ID: (Mouse) 12978
microglial cell activation
neutrophil homeostasis
positive regulation of protein phosphorylation
myeloid leukocyte differentiation
response to ischemia
response to stress
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
axon guidance
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
anatomical structure morphogenesis
positive regulation of gene expression
positive regulation of macrophage chemotaxis
negative regulation of myotube differentiation
microglia development
cell migration
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
telencephalon development
dentate gyrus development
olfactory bulb development
forebrain neuron differentiation
regulation of cell morphogenesis
hemopoiesis
osteoclast differentiation
positive regulation of cell migration
positive regulation of bone mineralization
negative regulation of epithelial cell differentiation
ruffle organization
positive regulation of chemokine production
positive regulation of tumor necrosis factor production
regulation of mononuclear cell proliferation
regulation of actin cytoskeleton organization
monocyte homeostasis
cellular response to macrophage colony-stimulating factor stimulus
macrophage colony-stimulating factor signaling pathway
positive regulation of tyrosine phosphorylation of STAT protein
negative regulation of apoptotic process
regulation of MAPK cascade
positive regulation by host of viral process
regulation of bone resorption
cell-cell junction maintenance
positive regulation of osteoclast differentiation
protein autophosphorylation
positive regulation of lymphocyte proliferation
positive regulation of protein kinase B signaling
positive regulation of protein tyrosine kinase activity
microglial cell proliferation
positive regulation of astrocyte activation
positive regulation of ERK1 and ERK2 cascade
positive regulation of leukocyte proliferation
cellular response to cytokine stimulus
positive regulation of macrophage proliferation
positive regulation of cell cycle G1/S phase transition
negative regulation of platelet formation
regulation of macrophage migration
positive regulation of macrophage migration
neuron projection extension
positive regulation of cell motility
positive regulation of myoblast proliferation
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