In-Cell ELISA Colorimetric Detection Kit

Catalog number: 62200

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific In-Cell ELISA Colorimetric Detection Kit is a simple and convenient method for quantification of intracellular protein levels in whole cells.

Features of the In-Cell ELISA Colorimetric Detection Kit:

• Colorimetric—compatible with standard ELISA plate readers
• Simple, fast assay format
• Ability to perform multiplex experiments
• No special sample preparation required
• Higher throughput compared with western blotting
• Cost efficient (one well vs. one lane) compared to western blotting

While traditional western blot analysis is time consuming and only semi-quantitative, the in-cell ELISA is an accurate method to determine relative protein levels and degree of post-translational modification (PTM) among various cell types. The In-cell ELISA may be performed in 96- or 384-well microplates, is scalable and conserves cell culture and treatment reagents. Because assay data are measured using a standard ELISA plate reader or infrared imaging system, the results are readily available for analysis. Also, the assay is amenable to automation, which is ideal for siRNA studies and drug screens.

Applications:
• High throughput determination of relative protein expression levels
• Monitor dose-dependent post-translational protein modification
• Multiplex analysis of targets in multiple cell lines

The In-Cell ELISA Colorimetric Detection Kit enables the levels of target proteins to be compared in different wells. The relative amount of protein is determined using target-specific primary antibodies and a horseradish peroxidase (HRP)-conjugated detection reagent. Following the colorimetric measurement of HRP activity, the provided whole cell stain, Janus Green, is used to determine cell number. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences.

Related Products
In-Cell ELISA Near Infrared Detection Kit
Janus Green Whole-Cell Stain for In-Cell ELISA Colorimetric Detection Kit
In-Cell ELISA Stripping Buffer
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 4 x 96 test kit
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Sufficient For: 4 x 96-well microplates of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Tween-20 Detergent), 10 mL
• Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 mL
• HRP Conjugate, 0.15 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each

Store antibodies as indicated on vials. Store remaining components at 4°C.

Documents

Manuals & protocols