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Thermo Scientific™

M-PER™ Mammalian Protein Extraction Reagent

Catalog number: 78501
Thermo Scientific™

M-PER™ Mammalian Protein Extraction Reagent

Catalog number: 78501
Catalog Number
78501
Unit Size
250 mL
Price (USD)
Price: 343.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
25 mL
Price (USD)
Price: 90.50
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 L
Price (USD)
Price: 890.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
78501250 mL
Price: 343.00
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7850325 mL
Price: 90.50
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785051 L
Price: 890.00
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Thermo Scientific M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total soluble protein extraction from cultured mammalian cells.

Features of M-PER Mammalian Protein Extraction Reagent:

Gentle—mild detergent lysis, yielding extracts that are immediately compatible with coomassie (Bradford) and BCA protein assays or SDS-PAGE
Compatible—extracts soluble proteins in nondenatured state, enabling direct use in immunoprecipitation and other affinity purification procedures
Amine-free and dialyzable—formulation ensures compatibility with subsequent assay systems
Convenient—lyse adherent cells directly in plate or after scraping and washing in suspension
Non-denaturing—maintain activity of luciferase, beta-galactosidase, CAT and other reporter genes as well or better than other suppliers' products and freeze/thaw methods

Product Details

The complete cell lysis reagent contains a mild, nondenaturing detergent that dissolves cell membranes to extract and solubilize total protein from most cellular compartments. Extraction is accomplished in only 5 minutes and requires little or no additional mechanical disruption. M-PER reagent is formulated for minimal interference with downstream biological applications. The reagent has been validated for use with several cell lines, including primary, suspension and adherent cell types; the resulting cell lysates are compatible with many downstream assays including immunoassays, enzyme assays and a variety of common reporter assays.

Cellular protein extraction—cell lysis to release the proteins of interest—is a key first step in many proteomics analysis procedures. M-PER reagent is designed to efficiently extract soluble protein from a variety of cell types, including primary cells and cells grown in suspension or adherent culture conditions. Performance of M-PER reagent was evaluated for yields of both total protein and specific target proteins from the various cellular compartments. Additionally, because M-PER reagent is formulated with dialyzable components that are devoid of primary amines, it is more compatible with certain downstream applications, such as luciferase, beta-galactosidase, and CAT assays, than other protein extraction methods.

Related Products
T-PER™ Tissue Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
M-PER™
Quantity
250 mL
Format
Liquid
Product Type
Protein Extraction Reagent
Volume (Metric)
250 mL

Contents & Storage

Upon receipt store product at room temperature.

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