The Thermo Scientific Pierce Magnetic HA-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.
Features of the HA-Tag Magnetic IP/Co-IP Kit:
• Specific magnetic beads—covalently immobilized high-quality anti-HA monoclonal antibody enables high yields of immunoprecipitation products • Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding • Trouble-free elution—low-pH elution buffer ensures recovery of HA-tagged protein interaction complexes without antibody leaching contamination • Convenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hour • Versatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.g., Thermo Scientific KingFisher Instruments)
Unlike traditional IP procedures based on capture with Protein A/G beads, this kit uses magnetic beads containing pre-immobilized anti-HA antibody. These Pierce Anti-HA Magnetic Beads ensure specific binding of HA-tagged protein complexes from biological samples. Because the antibody is covalently attached to the beads, IP-targets are easily eluted and recovered without antibody contamination. The complete IP kit includes the magnetic beads, lysis/wash buffer, low-pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer for SDS-PAGE. Protocols are provided for both manual and automated magnetic separation workflows. Sufficient components are provided to perform 40 IP or co-IP assays.
The hemagglutinin (HA) peptide (YPYDVPDYA), derived from the human influenza virus HA protein, is one of several fusion protein tags used for recombinant protein expression. The Pierce Magnetic HA-Tag IP/Co-IP Kit uses a specific, high-affinity immobilized antibody (clone 2-2.2.14) for rapid purification of HA-tagged fusion proteins from bacterial and mammalian cell lysates, as well as from lysates prepared with the Pierce Human in vitro Translation Kits. The beads are incubated with a cell lysate containing HA-tagged protein, the fusion protein is captured, and the beads are subsequently washed and then eluted using low-pH elution buffer or non-reducing sample buffer. The protocol and buffers have been optimized for both IP and co-IP reactions, enriching for specific protein interaction complexes in the eluted samples. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Equipment):
KingFisher™ Flex, Magnetic Stand
Pull-Down (Tag-Based), Immunoprecipitation (IP)
Contents & storage
Sufficient For: 40 IP reactions using 25 µL of anti-HA magnetic beads • HA-tagged Positive Control (Part No. 26180X), 500 µL • Pierce Anti-HA Magnetic Beads, 1 mL • Pierce IP Lysis/Wash Buffer, 2 × 50 mL • Non-Reducing Sample Buffer (5X), 5 mL • Elution Buffer (pH 2.0), 5 mL • Neutralization Buffer (pH 8.5), 1 mL
HA-tagged Positive Control ships separately—store at -70°C. Store remaining components at 4°C.