Invitrogen™

GeneArt™ Linear pUC19L Vector for Seamless Cloning

Catalog number:  A13289

Related applications:

PCR Cloning

| Seamless Cloning & Genetic Assembly

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Description

The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.

Some of the key features and elements of the pUC19L vector include:
lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt® Seamless Cloning and Assembly Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cloning Method: Seamless Cloning
Maximum Size: 13 kb total (vector plus all inserts)
Number of Fragments: Up to 4 Fragments
Product Line: GeneArt®
Replication Origin: pUC Origin
Shipping Condition: Dry Ice
Structure: Linear
Reporter Gene: Beta-Gal (lacZ)
Vector: Seamless Cloning Vectors

Contents & storage

The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt® Seamless Cloning and Assembly Kit (A13288 - must be purchased separately).

Store the product at -80°C.

The vector is guaranteed stable for 6 months when properly stored.