Invitrogen™
CSM Media for Mav203 Yeast Cells
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Description
The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g
Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).
Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Bacterial or Yeast Strain: | MaV203 |
|---|---|
| Compatible Products: | Optimized for use with the GENEART® High-Order Genetic Assembly System, A13285 |
| Product Line: | GeneArt® |
| Sterility: | Nonsterile |
| Target Organism Class: | S. cerevisiae |
| Form: | Liquid, Powder |
| Final Product: | Agar Plates |
| Preparation Method: | Autoclave |
| Product Size: | 2 L |
| Shipping Condition: | Room Temperature |
Contents & storage
CSM Media for MaV203 Yeast Cells has enough reagents to prepare 2 liters of agar media. The product contains two pouches of premixed powder and two 100 ml bottles of filter-sterilized, 20% glucose.
Store all components at room temperature.
All components are guaranteed stable for 6 months when properly stored.
Store all components at room temperature.
All components are guaranteed stable for 6 months when properly stored.
