CSM Media for Mav203 Yeast Cells

Catalog number: A13292

Invitrogen™  Related applications: Seamless Cloning & Genetic Assembly | Transformation

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Description

The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:

• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g

Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).

Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: MaV203
Compatible Products: Optimized for use with the GENEART® High-Order Genetic Assembly System, A13285
Sterility: Nonsterile
Target Organism Class: S. cerevisiae
Form: Liquid, Powder
Final Product: Agar Plates
Preparation Method: Autoclave
Product Size: 2 L
Shipping Condition: Room Temperature

Contents & storage

CSM Media for MaV203 Yeast Cells has enough reagents to prepare 2 liters of agar media. The product contains two pouches of premixed powder and two 100 ml bottles of filter-sterilized, 20% glucose.

Store all components at room temperature.

All components are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols