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Gibco™
Expi293™ Expression Medium
Description
Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red
Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Product use
Customers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Line: | 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F |
|---|---|
| Classification: | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Culture Environment: | CO2 |
| Endotoxin Level: | < 1 EU/ml |
| Format: | Bottle(s) |
| Glucose: | High Glucose |
| HEPES Buffer: | No HEPES |
| Included Antibiotics: | No antibiotics |
| Inorganic Salts: | Calcium, Magnesium, Sodium Phosphate |
| Osmolality: | 275-295 mOsm/kg |
| Product Line: | Expi293™ |
| Purity or Quality Grade: | Cell Culture Grade |
| Serum Level: | Serum-Free |
| Sodium Bicarbonate Buffer: | Sodium Bicarbonate |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Supplements: | No Hypoxanthine, No Thymidine, Insulin-Free, No Vitamin A, No 2-Mercaptoethanol, Dextrose, Pyridoxine HCl |
| Cell Type: | Kidney (Embryonic) |
| Culture Type: | Suspension Cell Culture |
| Form: | Liquid |
| Glutamine: | GlutaMAX™-I |
| Phenol Red Indicator: | No Phenol Red |
| Concentration: | 1 X |
| Sterility: | Sterile |
Contents & storage
Store in the dark at +2°C to +8°C
