Expi293™ Expression Medium

Catalog number: A1435102

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture | Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: 293 (HEK), 293E (HEK), 293F, Expi293F™, FreeStyle™ 293-F Cells
Cell Type: Kidney (Embryonic)
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Concentrated: 1 X
Culture Environment: CO2
Culture Type: Suspension Cell Culture
Endotoxin Level: < 1 EU/ml
Form: Liquid
Format: Bottle(s)
Glucose: High Glucose
Glutamine: GlutaMAX™-I
HEPES Buffer: No HEPES
Included Antibiotics: No antibiotics
Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
Osmolality: 275-295 mOsm/kg
Phenol Red Indicator: No Phenol Red
Product Size: 6 x 1 L
Purity or Quality Grade: Cell Culture Grade
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Sterility: Sterile
Supplements: Dextrose, Insulin-Free, No 2-Mercaptoethanol, No Hypoxanthine, No Thymidine, No Vitamin A, Pyridoxine HCl

Contents & storage

Store in the dark at +2°C to +8°C

Documents

Manuals & protocols

Recommended products