Expi293™ Expression Medium

Description

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Specifications

Form:	Liquid
Format:	Bottle(s)
Glucose:	High Glucose
Cell Line:	293E (HEK), 293 (HEK), 293F, Expi293F™, FreeStyle™ 293-F Cells
Cell Type:	Kidney (Embryonic)
Glutamine:	GlutaMAX™-I
Sterility:	Sterile
Osmolality:	275-295 mOsm/kg
Serum Level:	Serum-Free
Supplements:	No Hypoxanthine, Insulin-Free, No 2-Mercaptoethanol, No Vitamin A, Dextrose, Pyridoxine HCl, No Thymidine
Concentrated:	1 X
Culture Type:	Suspension Cell Culture
HEPES Buffer:	No HEPES
Product Size:	6 x 1 L
Classification:	Animal Origin-Free, Protein-Free, Serum-Free, Chemically Defined
Endotoxin Level:	< 1 EU/ml
Inorganic Salts:	Sodium Phosphate, Calcium, Magnesium
Culture Environment:	CO2
Included Antibiotics:	No antibiotics
Phenol Red Indicator:	No Phenol Red
Purity or Quality Grade:	Cell Culture Grade
Sodium Pyruvate Additive:	Sodium Pyruvate
Sodium Bicarbonate Buffer:	Sodium Bicarbonate

Contents & storage