Veriti™ Thermal Cycler Extended Warranty Package, 96-well Fast, System + 3 yr REX ext warranty - FAQs

View additional product information for Veriti™ Thermal Cycler, 96-well Fast - FAQs (A27924)

2 product FAQs found

Have you tested the Veriti and VeritiPro Thermal Cyclers in low humidity conditions (below 20%)?

The recommended conditions are determined to ensure no static discharge (low humidity) occurs.
Recommended Conditions:
- Veriti: 20-80% humidity
- VeritiPro: 15-80% humidity
We have not tested Veriti and VeritiPro thermal cyclers in low humidity, below 20%, therefore we cannot recommend using them in those conditions.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.