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Bethyl Laboratories
HRS Polyclonal Antibody
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FIGURE: 1 / 1
HRS Antibody (A300-988A) in IP
Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HRS antibody (Product # A300-988A) used for IP at 3 µg/mg lysate. HRS was also immunoprecipitated by rabbit anti-HRS antibody A300-989A, which recognizes a downstream epitope. For blotting immunoprecipitated HRS, A300-989A was used at 1 µg/mL,. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-988A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 350 and 400 of human growth factor-regulated tyrosine kinase substrate.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hepatocyte growth factor-regulated tyrosine kinase substrate; HGNC:4897; Hrs; human growth factor-regulated tyrosine kinase substrate; Protein pp110; unnamed protein product
Gene Aliases: HGS; HRS
UniProt ID: (Human) O14964
Entrez Gene ID: (Human) 9146
protein targeting to lysosome
signal transduction
negative regulation of cell proliferation
membrane invagination
positive regulation of gene expression
negative regulation of platelet-derived growth factor receptor signaling pathway
protein transport
endosomal transport
macroautophagy
negative regulation of angiogenesis
negative regulation of vascular endothelial growth factor receptor signaling pathway
receptor internalization
endocytic recycling
multivesicular body assembly
regulation of protein catabolic process
protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of MAP kinase activity
negative regulation of JAK-STAT cascade
protein localization to membrane
membrane fission
positive regulation of exosomal secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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