Gibco™ PSC Definitive Endoderm Induction Kit consists of two xeno-free media that enable efficient induction of human pluripotent stem cells (PSCs) to definitive endoderm. Unlike other methods that require multiple components and take five or more days, the PSC Definitive Endoderm Induction Kit can generate ≥90% CXCR4+/PDGFRα- definitive endoderm cells with only two components in just two days. Each medium is supplied as a 1X complete medium, requiring no mixing in of additional components, and the resultant definitive endoderm is capable of differentiating into midgut/hindgut, pancreatic endoderm, or liver bud progenitors that express relevant physiological markers.
Generation of high quality definitive endoderm
Definitive endoderm generated using the PSC Definitive Endoderm Induction Kit is capable of differentiating into downstream lineages such as midgut/hindgut (expressing CDX2), pancreatic endoderm (expressing PDX2), and liver bud progenitors (expressing AFP).
Rapid production of definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing definitive endoderm in only two days.
Generation of highly pure definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing highly pure populations of definitive endoderm from pluripotent stem cells, showing ≥90% CXCR4+/PDGFRα- across multiple lines and high expression of key markers SOX17 and FOXA2.
No mixing in of components required
Each medium is supplied at a 1X concentration, requiring no mixing in of reagents. Simply thaw the media and add to PSC culture.