Gibco™

ExpiFectamine™ Sf Transfection Reagent

Catalog number:  A38915

 Related applications:

Protein Expression

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Description

ExpiFectamine Sf Transfection Reagent is a next-generation cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.

ExpiFectamine Sf Transfection Reagent:
• Delivers robust and reproducible transfection, giving you greater confidence in your results
• Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
• Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• Exhibits low toxicity and does not require medium change post-transfection
• Is part of a simple, fast protocol with a short 5-minute complexation time
• Demonstrates linear scalability of transfections from culture volumes of 4 mL to 500 mL in suspension format

The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9 Cells. However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Insect Cells
Sample Type (Specific): Baculovirus DNA, Plasmid DNA
Transfection Technique: Lipid-Based Transfection
Serum Compatible: Yes
High Throughput Compatibility: High Throughput-Compatible
Product Line: ExpiFectamine™
Product Size: 1 mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Store at 2–8°C, do not freeze

Shelf Life: 24 months from date of manufacture

Documents

Manuals & protocols